Directory: Top/Science/Biology/Biochemistry_and_Molecular_Biology/Methods_and

Top / Science / Biology / Biochemistry_and_Molecular_Biology / Methods_and_Techniques / PCR /

Roe Laboratory Protocols
Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
http://www.genome.ou.edu/proto.html
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FISH Guide and Troubleshooting
Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT.
http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html
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The web guide of PCR
List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.
http://www.pcrlinks.com/
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Long PCR Protocol
Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html
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HiFi DNA
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
http://www.hifidna.com/
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PCR Project
Presentations from the University of California at Berkley on PCR, both current research reports and reviews.
http://sunsite.berkeley.edu/biotech/pcr/
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PCR Protocols
Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html
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PCR Technology
An introduction by Connie Veilleux from the US National Health Museum website.
http://www.accessexcellence.org/LC/SS/PS/PCR/PCR_technology.html
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Polymerase Chain Reaction (PCR)
A graphic description of the principle of PCR from the US National Health Museum web site.
http://www.accessexcellence.org/AB/GG/polymerase.html
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Rep-PCR Genomic Fingerprinting
Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.
http://www.msu.edu/user/debruijn/
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